A SIMPLE INTRAPIPETTE SALT BRIDGE

被引：7

作者：

KLEENE, SJ

机构：

[1] Department of Anatomy and Cell Biology, University of Cincinnati, Cincinnati

来源：

JOURNAL OF NEUROSCIENCE METHODS | 1993年 / 46卷 / 01期

关键词：

SALT BRIDGE; PATCH-CLAMP; ELECTROPHYSIOLOGY; CHLORIDE;

D O I：

10.1016/0165-0270(93)90136-F

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A simple method is described for achieving a salt bridge within a patch pipette. The tip of the pipette is filled with a Cl--free solution that bathes the membrane patch. Above this is a Cl--containing solution that surrounds the recording Ag/AgCl wire electrode. Addition of 0.07% (w/v) agarose to the solution in the tip prevents bulk mixing of the 2 phases within the pipette. This configuration makes it possible to remove Cl- from the membrane surface but maintain it at the electrode surface. The presence of the Cl--free phase in the bottom of the patch pipette does not reduce the electrical stability of the recording electrode.

引用

页码：11 / 16

页数：6

共 10 条

[1] LIQUID JUNCTION POTENTIALS AND SMALL-CELL EFFECTS IN PATCH-CLAMP ANALYSIS [J].