REGIONAL BRAIN EXPRESSION OF SEROTONIN TRANSPORTER MESSENGER-RNA AND ITS REGULATION BY REUPTAKE INHIBITING ANTIDEPRESSANTS

被引：208

作者：

LESCH, KP ^{[1
]}

AULAKH, CS ^{[1
]}

WOLOZIN, BL ^{[1
]}

TOLLIVER, TJ ^{[1
]}

HILL, JL ^{[1
]}

MURPHY, DL ^{[1
]}

机构：

[1] NIMH,CTR CLIN,CLIN SCI LAB,NEUROPHARMACOL SECT,BETHESDA,MD 20892

来源：

MOLECULAR BRAIN RESEARCH | 1993年 / 17卷 / 1-2期

关键词：

SEROTONIN TRANSPORTER MESSENGER RNA; POLYMERASE CHAIN REACTION; REGIONAL BRAIN EXPRESSION; ANTIDEPRESSANT DRUG;

D O I：

10.1016/0169-328X(93)90069-2

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

Regional expression and antidepressant drug-induced regulation of mRNA encoding the serotonin (5-HT) transporter were studied in rat brain. While 5-HT transporter mRNA is abundantly expressed in the midbrain raphe complex, lower concentrations were also found in frontal cortex, hippocampus, and neostriatum using a combination of reverse transcriptase-polymerase chain reaction (RT-PCR), Southern hybridization, and sequence analysis. Long-term administration of antidepressants which inhibit 5-HT reuptake, but not monoamine oxidase inhibitors or 5-HT receptor agonists, decrease 5-HT transporter mRNA steady-state concentrations. Based on these observations, we conclude that (1) mRNA coding for the 5-HT transporter is present in several brain areas associated with ascending HT pathways, and (2) chronic treatment with reuptake inhibiting antidepressants may be associated with regulation of the 5-HT transporter at the level of gene expression which may contribute to the neuroadaptive mechanisms that likely underlie their therapeutic efficacy.

引用

页码：31 / 35

页数：5

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