CRITICAL STRUCTURAL ELEMENTS OF THE VP16 TRANSCRIPTIONAL ACTIVATION DOMAIN

被引：407

作者：

CRESS, WD ^{[1
]}

TRIEZENBERG, SJ ^{[1
]}

机构：

[1] MICHIGAN STATE UNIV,DEPT BIOCHEM,E LANSING,MI 48824

来源：

SCIENCE | 1991年 / 251卷 / 4989期

关键词：

D O I：

10.1126/science.1846049

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Virion protein 16 (VP16) of herpes simplex virus type 1 contains an acidic transcriptional activation domain. Missense mutations within this domain have provided insights into the structural elements critical for its function. Net negative charge contributed to, but was not sufficient for, transcriptional activation by VP16. A putative amphipathic alpha helix did not appear to be an important structural component of the activation domain. A phenylalanine residue at position 442 was exquisitely sensitive to mutation. Transcriptional activators of several classes contain hydrophobic amino acids arranged in patterns resembling that of VP16. Therefore, the mechanism of transcriptional activation by VP16 and other proteins may involve both ionic and specific hydrophobic interactions with target molecules.

引用

收藏

页码：87 / 90

页数：4

相关论文

共 39 条

[1] MUTATIONAL ANALYSIS OF THE HERPES-SIMPLEX VIRUS TYPE-1 TRANS-INDUCING FACTOR VMW65 [J].

ACE, CI ;

DALRYMPLE, MA ;

RAMSAY, FH ;

PRESTON, VG ;

PRESTON, CM .

JOURNAL OF GENERAL VIROLOGY, 1988, 69 :2595-2605

[2] SELECTIVE-INHIBITION OF ACTIVATED BUT NOT BASAL TRANSCRIPTION BY THE ACIDIC ACTIVATION DOMAIN OF VP16 - EVIDENCE FOR TRANSCRIPTIONAL ADAPTERS [J].

BERGER, SL ;

CRESS, WD ;

CRESS, A ;

TRIEZENBERG, SJ ;

GUARENTE, L .

CELL, 1990, 61 (07) :1199-1208

[3] IDENTIFICATION OF HERPES-SIMPLEX VIRUS-DNA SEQUENCES WHICH ENCODE A TRANS-ACTING POLYPEPTIDE RESPONSIBLE FOR STIMULATION OF IMMEDIATE EARLY TRANSCRIPTION [J].

CAMPBELL, MEM ;

PALFREYMAN, JW ;

PRESTON, CM .

JOURNAL OF MOLECULAR BIOLOGY, 1984, 180 (01) :1-19

[4] ANALYSIS OF SP1 INVIVO REVEALS MULTIPLE TRANSCRIPTIONAL DOMAINS, INCLUDING A NOVEL GLUTAMINE-RICH ACTIVATION MOTIF [J].

COUREY, AJ ;

TJIAN, R .

CELL, 1988, 55 (05) :887-898

[5] THE C-TERMINAL 79 AMINO-ACIDS OF THE HERPES-SIMPLEX VIRUS REGULATORY PROTEIN, VMW65, EFFICIENTLY ACTIVATE TRANSCRIPTION IN YEAST AND MAMMALIAN-CELLS IN CHIMERIC DNA-BINDING PROTEINS [J].

COUSENS, DJ ;

GREAVES, R ;

GODING, CR ;

OHARE, P .

EMBO JOURNAL, 1989, 8 (08) :2337-2342

[6]

CRESS WD, UNPUB

[7] A COMPREHENSIVE SET OF SEQUENCE-ANALYSIS PROGRAMS FOR THE VAX [J].

DEVEREUX, J ;

HAEBERLI, P ;

SMITHIES, O .

NUCLEIC ACIDS RESEARCH, 1984, 12 (01) :387-395

[8] IDENTIFICATION AND CHARACTERIZATION OF HAP4 - A 3RD COMPONENT OF THE CCAAT-BOUND HAP2 HAP3 HETEROMER [J].

FORSBURG, SL ;

GUARENTE, L .

GENES & DEVELOPMENT, 1989, 3 (08) :1166-1178

[9] A HERPESVIRUS TRANS-ACTIVATING PROTEIN INTERACTS WITH TRANSCRIPTION FACTOR OTF-1 AND OTHER CELLULAR PROTEINS [J].

GERSTER, T ;

ROEDER, RG .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1988, 85 (17) :6347-6351

[10] TRANSCRIPTION IN YEAST ACTIVATED BY A PUTATIVE AMPHIPATHIC ALPHA-HELIX LINKED TO A DNA-BINDING UNIT [J].

GINIGER, E ;

PTASHNE, M .

NATURE, 1987, 330 (6149) :670-672

← 1 2 3 4 →