MICROASSAY FOR DNA METHYLTRANSFERASE

被引：55

作者：

ADAMS, RLP

RINALDI, A

SEIVWRIGHT, C

机构：

[1] Department of Biochemistry, University of Glasgow, Glasgow

来源：

JOURNAL OF BIOCHEMICAL AND BIOPHYSICAL METHODS | 1991年 / 22卷 / 01期

基金：

英国医学研究理事会;

关键词：

DNA; METHYLTRANSFERASE; METHYLASE; MICROASSAY;

D O I：

10.1016/0165-022X(91)90077-A

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A microassay for DNA methylase is described which can detect activity in as few as 50 tissue culture cells. The cells are lysed and incubated for 2 h at 37-degrees-C with 3-mu-Ci high specific activity [H-3]AdoMet and 0.5-mu-g poly]d(I-C).d(I-C)] in a volume of 23-mu-1. Ribonuclease is present during the assay and the product DNA is isolated by phenol extraction after protease digestion.

引用

页码：19 / 22

页数：4

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