AN ENDOPLASMIC RETICULUM-SPECIFIC CYCLOPHILIN

被引：128

作者：

HASEL, KW ^{[1
]}

GLASS, JR ^{[1
]}

GODBOUT, M ^{[1
]}

SUTCLIFFE, JG ^{[1
]}

机构：

[1] Scripps Res Inst, RES INST, DEPT MOLEC BIOL, 10666 N TORREY PINES RD, LA JOLLA, CA 92037 USA

来源：

MOLECULAR AND CELLULAR BIOLOGY | 1991年 / 11卷 / 07期

关键词：

D O I：

10.1128/MCB.11.7.3484

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Cyclophilin is a ubiquitously expressed cytosolic peptidyl-prolyl cis-trans isomerase that is inhibited by the immunosuppressive drug cyclosporin A. A degenerate oligonucleotide based on a conserved cyclophilin sequence was used to isolate cDNA clones representing a ubiquitously expressed mRNA from mice and humans. This mRNA encodes a novel 20-kDa protein, CPH2, that shares 64 % sequence identity with cyclophilin. Bacterially expressed CPH2 binds cyclosporin A and is a cyclosporin A-inhibitable peptidyl-prolyl cis-trans isomerase. Cell fractionation of rat liver followed by Western blot (immunoblot) analysis indicated that CPH2 is not cytosolic but rather is located exclusively in the endoplasmic reticulum. These results suggest that cyclosporin A mediates its effect on cells through more than one cyclophilin and that cyclosporin A-induced misfolding of T-cell membrane proteins normally mediated by CPH2 plays a role in immunosuppression.

引用

页码：3484 / 3491

页数：8

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