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AN IMPROVED TECHNIQUE FOR SEPARATION OF GLUCOSE 6-PHOSPHATE DEHYDROGENASE ISOENZYMES BY DISC ELECTROPHORESIS ON POLYACRYLAMIDE GEL

被引：39
作者：
BAKAY, B
NYHAN, WL
机构：
[1] Departments of Pediatrics and Biochemistry, University of Miami School of Medicine, Miami, Florida
来源：
BIOCHEMICAL GENETICS | 1969年 / 3卷 / 06期
关键词：
D O I：
10.1007/BF00485478
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
A new method has been developed for the separation of glucose 6-phosphate dehydrogenase (G6PD) isoenzymes by means of disc electrophoresis. The effects of gel concentration, method of preparation, and application of samples on the separation of types A and B enzymes and hemoglobin were examined. The method not only separated the isoenzymes but also subresolved each isoenzyme into subbands. The formation of subbands may be related to the existence of enzyme subunits. © 1969 Plenum Publishing Corporation.
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页码：571 / &
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