CLONING AND EXPRESSION OF HUMAN ENDOTHELIN-1 RECEPTOR CDNA

被引：281

作者：

HOSODA, K

NAKAO, K

HIROSHIARAI

SUGA, S

OGAWA, Y

MUKOYAMA, M

SHIRAKAMI, G

SAITO, Y

NAKANISHI, S

IMURA, H

机构：

[1] KYOTO UNIV,SCH MED,DEPT MED,DIV 2,54 SHOGOIN KAWAHARA CHO,SAKYO KU,KYOTO 606,JAPAN

[2] KYOTO UNIV,SCH MED,INST IMMUNOL,KYOTO 606,JAPAN

来源：

FEBS LETTERS | 1991年 / 287卷 / 1-2期

关键词：

ENDOTHELIN (ET); RECEPTOR; CDNA CLONING; G-PROTEIN; XENOPUS-OOCYTE; NORTHERN BLOT HYBRIDIZATION ANALYSIS;

D O I：

10.1016/0014-5793(91)80007-P

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We isolated a human endothelin-1 (ET-1) receptor cDNA from a human placenta cDNA library. The cDNA encodes a 427-amino acid protein with seven putative transmembrane domains. The rank order of the binding to the receptor expressed in COS-7 cells was: ET-1 greater-than-or-equal-to ET-2 >> ET-3. The receptor expressed in Xenopus oocytes showed a potent electrophysiological response to 1 x 10(-7) M ET-1 under voltage clamp at -60 mV, while a much weaker response was produced by 1 x 10(-7) M ET-3. Northern blot analysis with RNA from human tissues revealed a single band with a size of 4.3 kb in a wide variety of human tissues, especially highly in the blood vessel.

引用

页码：23 / 26

页数：4

共 22 条

[1] CLONING AND EXPRESSION OF A CDNA-ENCODING AN ENDOTHELIN RECEPTOR [J].