FACILE PREPARATION OF 3'-OLIGONUCLEOTIDE-PEPTIDE CONJUGATES

被引：55

作者：

JUBY, CD ^{[1
]}

RICHARDSON, CD ^{[1
]}

BROUSSEAU, R ^{[1
]}

机构：

[1] NATL RES COUNCIL CANADA,BIOTECHNOL RES INST,GENET ENGN SECT,MONTREAL H4P 2R2,QUEBEC,CANADA

来源：

TETRAHEDRON LETTERS | 1991年 / 32卷 / 07期

关键词：

D O I：

10.1016/S0040-4039(00)92110-5

中图分类号：

O62 [有机化学];

学科分类号：

070303 ; 081704 ;

摘要：

The solid phase synthesis of 3' deoxyoligonucleotide (17 mer)-peptide conjugates is described. The oligonucleotide is complementary to the template strand of the measles virus in the region of the nucleocapsid protein on the 60 nucleotide leader RNA. The conjugates were synthesized on teflon support using a combination of solid phase FMOC peptide chemistry and phosphoramidite chemistry linked by a branched modifier.

引用

页码：879 / 882

页数：4

共 12 条

[1] SYNTHESIS OF ALKYLATING OLIGONUCLEOTIDE DERIVATIVES CONTAINING CHOLESTEROL OR PHENAZINIUM RESIDUES AT THEIR 3'-TERMINUS AND THEIR INTERACTION WITH DNA WITHIN MAMMALIAN-CELLS [J].

BOUTORIN, AS ;

GUSKOVA, LV ;

IVANOVA, EM ;

KOBETZ, ND ;

ZARYTOVA, VF ;

RYTE, AS ;

YURCHENKO, LV ;

VLASSOV, VV .

FEBS LETTERS, 1989, 254 (1-2) :129-132

[2] TOPOGRAPHY OF NUCLEIC-ACID HELICES IN SOLUTION .26. SPECIFIC INTERACTION OF PEPTIDES WITH NUCLEIC-ACIDS [J].

GABBAY, EJ ;

BAXTER, CS ;

SANFORD, K .

BIOCHEMISTRY, 1972, 11 (18) :3429-&

[3] THE SYNTHESIS OF POLYAMIDE OLIGONUCLEOTIDE CONJUGATE MOLECULES [J].

HARALAMBIDIS, J ;

DUNCAN, L ;

ANGUS, K ;

TREGEAR, GW .

NUCLEIC ACIDS RESEARCH, 1990, 18 (03) :493-499

[4] PREPARATION OF BASE-MODIFIED NUCLEOSIDES SUITABLE FOR NONRADIOACTIVE LABEL ATTACHMENT AND THEIR INCORPORATION INTO SYNTHETIC OLIGODEOXYRIBONUCLEOTIDES [J].

HARALAMBIDIS, J ;

CHAI, M ;

TREGEAR, GW .

NUCLEIC ACIDS RESEARCH, 1987, 15 (12) :4857-4876

[5] SPECIFIC REGULATION OF GENE-EXPRESSION BY ANTISENSE, SENSE AND ANTIGENE NUCLEIC-ACIDS [J].

HELENE, C ;

TOULME, JJ .

BIOCHIMICA ET BIOPHYSICA ACTA, 1990, 1049 (02) :99-125

[6] SPECIFIC ANTIVIRAL ACTIVITY OF A POLY(L-LYSINE)-CONJUGATED OLIGODEOXYRIBONUCLEOTIDE SEQUENCE COMPLEMENTARY TO VESICULAR STOMATITIS-VIRUS N-PROTEIN MESSENGER-RNA INITIATION SITE [J].

LEMAITRE, M ;

BAYARD, B ;

LEBLEU, B .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1987, 84 (03) :648-652

[7]

LOHRMANN R, 1984, DNA-J MOLEC CELL BIO, V3, P122

[8] SOLID-PHASE SYNTHESES OF OLIGODEOXYRIBONUCLEOSIDE METHYLPHOSPHONATES [J].

MILLER, PS ;

REDDY, MP ;

MURAKAMI, A ;

BLAKE, KR ;

LIN, SB ;

AGRIS, CH .

BIOCHEMISTRY, 1986, 25 (18) :5092-5097

[9] SPECIFIC-INHIBITION OF PARAMYXOVIRUS AND MYXOVIRUS REPLICATION BY OLIGOPEPTIDES WITH AMINO-ACID-SEQUENCES SIMILAR TO THOSE AT THE N-TERMINI OF THE F1 OR HA2 VIRAL POLYPEPTIDES [J].

RICHARDSON, CD ;

SCHEID, A ;

CHOPPIN, PW .

VIROLOGY, 1980, 105 (01) :205-222

[10] OLIGODEOXYNUCLEOTIDES AS ANTI-SENSE INHIBITORS OF GENE-EXPRESSION - THERAPEUTIC IMPLICATIONS [J].

ROTHENBERG, M ;

JOHNSON, G ;

LAUGHLIN, C ;

GREEN, I ;

CRADOCK, J ;

SARVER, N ;

COHEN, JS .

JOURNAL OF THE NATIONAL CANCER INSTITUTE, 1989, 81 (20) :1539-1544

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