MOLECULAR RECOGNITION AT SELF-ASSEMBLED MONOLAYERS - THE CONSTRUCTION OF MULTICOMPONENT MULTILAYERS

被引：278

作者：

SPINKE, J

LILEY, M

GUDER, HJ

ANGERMAIER, L

KNOLL, W

机构：

[1] MAX PLANCK INST POLYMER RES,ACKERMANNWEG 10,W-6500 MAINZ,GERMANY

[2] BOEHRINGER MANNHEIM GMBH,WERK TUTZING,W-8132 TUTZING,GERMANY

来源：

LANGMUIR | 1993年 / 9卷 / 07期

关键词：

D O I：

10.1021/la00031a033

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

A multilayer system of proteins and organic thiols self-assembled on a gold surface, and with a different component in each layer, is investigated. The multilayer system chosen-biotinylated thiol; streptavidin; biotinylated Fab; human chorionic gonadotrophin; monoclonal antibody-is of relevance to immunoassays for pregnancy testing. The composition of the first thiol monolayer is shown to have a strong influence on the binding properties of all subsequent layers-not only in the efficiency of binding, but also in its specificity. The efficiencies of the binding processes between the different layers are analyzed. The sensitivity of the system to human chorionic gonadotrophin is measured and a detection limit of approximately 1 x 10(-8) M determined.

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页码：1821 / 1825

页数：5

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