SUCROSE-BINDING LECTIN IN REGENERATING COCKROACH (PERIPLANETA-AMERICANA) LEGS - ITS PURIFICATION FROM ADULT HEMOLYMPH

被引：30

作者：

KUBO, T

KAWASAKI, K

NATORI, S

机构：

[1] Faculty of Pharmaceutical Sciences, University of Tokyo, Bunkyo-ku, Tokyo

来源：

INSECT BIOCHEMISTRY | 1990年 / 20卷 / 06期

关键词：

hemolymph; immunoblotting; lectin; Periplaneta americana; regeneration;

D O I：

10.1016/0020-1790(90)90070-B

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Previously, we purified Periplaneta lectin from the hemolymph of adult Periplaneta americana (American cockroach) (Kubo and Natori Eur. J. Biochem. 168, 75-82, 1987). Immunoblotting analysis using antibody against Periplaneta lectin showed that the cockroach hemolymph contains another lectin that cross reacted immunologically with Periplaneta lectin. We have purified this new lectin (regenectin) to homogeneity. Affinity purified antibody against regenectin cross reacted with Periplaneta lectin. Thus, Periplaneta lectin and this new lectin were found to be different lectins sharing common antigenicity. After leg amputation, this new lectin was found to appear transiently at a specific stage of regeneration as revealed by immunoblotting, suggesting that it plays a role in the regeneration process. © 1990.

引用

页码：585 / 591

页数：7

共 29 条

[1] CARBOHYDRATE-SPECIFIC RECEPTORS OF THE LIVER [J].