RHODOPSIN INACTIVATION IS A MODULATED PROCESS IN LIMULUS PHOTORECEPTORS

被引:22
作者
RICHARD, EA
LISMAN, JE
机构
[1] Department of Biology, Brandeis University, Waltham
关键词
D O I
10.1038/356336a0
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
MANY G-protein-coupled receptors are only transiently active because an inactivation process stops the receptor from activating G protein molecules 1-3. Although this inactivated has been investigated in vitro, the real kinetics of the process can only be obtained from intact cells. Here we describe a method for measuring the inactivation of rhodopsin in intact photoreceptors and the application of this method to the ultraviolet rhodopsin of Limulus median eye 4. The results show that the inactivation process is very rapid (< 150 ms) and occurs well before the peak of the receptor potential. We have also investigated whether the inactivation process can itself be modulated. Our results show that light-adaptation accelerates inactivation by about 10-fold, providing evidence that G-protein-mediated transduction can be modulated at this first stage.
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页码:336 / 338
页数:3
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