PHOTOACTIVATED CONFORMATIONAL-CHANGES IN RHODOPSIN - A TIME-RESOLVED SPIN-LABEL STUDY

被引：144

作者：

FARAHBAKHSH, ZT

HIDEG, K

HUBBELL, WL

机构：

[1] UNIV CALIF LOS ANGELES,JULES STEIN EYE INST,LOS ANGELES,CA 90024

[2] UNIV CALIF LOS ANGELES,DEPT CHEM & BIOCHEM,LOS ANGELES,CA 90024

[3] UNIV PECS,CENT RES LAB,H-7643 PECS,HUNGARY

来源：

SCIENCE | 1993年 / 262卷 / 5138期

关键词：

D O I：

10.1126/science.8248781

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Rhodopsin has been selectively spin-labeled near the cytoplasmic termini of helices C and G. Photoactivation with a light flash induces an electron paramagnetic resonance spectral change in the millisecond time domain, coincident with the appearance of the active metarhodopsin II intermediate. The spectral change is consistent with a small movement near the cytoplasmic termination of the C helix and reverses upon formation of the MIII state. These results provide an important link between the optical changes associated with the retinal chromophore and protein conformational states.

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页码：1416 / 1419

页数：4

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