MITOCHONDRIAL AUTONOMY - INCORPORATION OF MONOSACCHARIDES INTO ENDOGENOUS GLYCOLIPID ACCEPTORS IN ISOLATED RAT LIVER MITOCHONDRIA

Isolated, sterile rat liver mitochondria homogenized in Triton X-100 incorporated glucose-14C and mannose-14C into endogenous lipid acceptors forming glycolipids. In addition, choline-14C was incorporated into phosphatidyl choline in the system. The glycolipid incorporations were highly specific and showed an absolute requirement for Mg++. One of the products formed with each monosaccharide was identified as a diglyceride glycolipid. Fractionation of the mitochondria into inner and outer membrane fractions indicated that the choline, glucose and mannose systems were associated with the outer membranes of the mitochondria. © 1969.