STRUCTURAL-CHANGES ACCOMPANYING GTP HYDROLYSIS IN MICROTUBULES - INFORMATION FROM A SLOWLY HYDROLYZABLE ANALOG GUANYLYL-(ALPHA,BETA)-METHYLENE-DIPHOSPHONATE

被引：191

作者：

HYMAN, AA ^{[1
]}

CHRETIEN, D ^{[1
]}

ARNAL, I ^{[1
]}

WADE, RH ^{[1
]}

机构：

[1] CNRS, CEA, INST BIOL STRUCT JEAN PIERRE EBEL, MICROSCOPIE ELECTR STRUCT LAB, F-38041 GRENOBLE 1, FRANCE

来源：

JOURNAL OF CELL BIOLOGY | 1995年 / 128卷 / 1-2期

关键词：

D O I：

10.1083/jcb.128.1.117

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

We have used cryoelectron microscopy to try to understand the structural basis for the role of GTP hydrolysis in destabilizing the microtubule lattice. We have measured a structural difference introduced into microtubules by replacing GTP with guanylyl-(alpha,beta)-methylene-diphosphonate (GMPCPP). In a stable GMPCPP microtubule lattice, the moire patterns change and the tubulin subunits increase in size by 1.5 Angstrom. This information provides a clue to the role of hydrolysis in inducing the structural change at the end of a microtubule during the transition from a growing to a shrinking phase.

引用

页码：117 / 125

页数：9

共 31 条

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