SELECTIVE RADIOCHEMICAL LABELING OF TYPE-A AND TYPE-B ACTIVE-SITES OF RAT-LIVER MONO-AMINE OXIDASE

被引：24

作者：

EDWARDS, DJ

PAK, KY

机构：

[1] Department of Psychiatry University, Pittsburgh School, Medicine Western Psychiatric Institute

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1979年 / 86卷 / 02期

关键词：

D O I：

10.1016/0006-291X(79)90872-6

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

By using clorgyline and deprenyl to block the types A and B active sites of monoamine oxidase, respectively, the remaining site could be selectively labeled radiochemically with [3H]pargyline. Titration of the A and B active sites in rat liver mitochondria indicated that they were present in a ratio of 1:3.3. Subunits containing the labeled A and B catalytic sites could not be separated by SDS-gel electrophoresis, and each had a molecular weight of 60,000 daltons. © 1979.

引用

页码：350 / 357

页数：8

共 17 条

[1] BIOCHEMICAL STUDIES OF SYNAPSES IN VITRO .I. PROTEIN SYNTHESIS [J].