RAPID SUBCHROMOSOMAL LOCALIZATION OF COSMIDS BY NONRADIOACTIVE INSITU HYBRIDIZATION

被引：173

作者：

KIEVITS, T

DAUWERSE, JG

WIEGANT, J

DEVILEE, P

BREUNING, MH

CORNELISSE, CJ

VANOMMEN, GJB

PEARSON, PL

机构：

[1] LEIDEN STATE UNIV,DEPT PATHOL,2333 AL LEIDEN,NETHERLANDS

[2] LEIDEN STATE UNIV,DEPT CYTOCHEM & CYTOMETRY,2333 AL LEIDEN,NETHERLANDS

来源：

CYTOGENETICS AND CELL GENETICS | 1990年 / 53卷 / 2-3期

关键词：

D O I：

10.1159/000132913

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

A rapid method for localizing large numbers of com-pleie cosmids by nonradioactive in situ hybridization is described. The cosmids arc nick translated in the presence of biotin-16- dl IF. mcubuled with an excess of sonicated human DNA. and used as a probe foi in Mlulivbiuh/ Snollu hridiaioiiy delected by successive treatments with FITC-labcled avidin and biolinylated unli-uvidin antihody. cosmids were localized on chromosome 16 in 5 d relative to translocation breakpoints contained in two cell lines Rapid identification of chromosome 16 was achieved by cohybridizalion with a chromosome 16 specific ccntromcric repeal probe. © 1990 S. Karger AG, Basel.

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页码：134 / &

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