SRC GENE-PRODUCT OF TRANSFORMED AND MORPHOLOGICALLY REVERTED ASV-INFECTED MAMMALIAN-CELLS

Morphological revertants of avian sarcoma virus transformed vole cells contain the sarcoma gene product (pp60src) in an enzymatically active form, suggesting that the presence of pp60src protein kinase activity is insufficient to induce morphological transformation. Structural analyses of pp60src from infected vole cell clones suggest that in one of the revertant clones an alteration in pp60src may be responsible for morphological reversion while in a second clone, reversion may result from an alteration in a cell gene product with which pp60src must interact. As these morphological revertant cells are tumorigenic, different cell components are required to interact with pp60src to facilitate the two events. © 1979 Nature Publishing Group.