SYNTHESIS OF A TRI-PHOSPHORYLATED PEPTIDE CORRESPONDING TO THE MAJOR AUTOPHOSPHORYLATION SITE IN THE INSULIN-RECEPTOR - CONFORMATIONAL COMPARISON WITH ITS NONPHOSPHORYLATED ANALOG

被引：5

作者：

CHAVANIEU, A ^{[1
]}

NAHARISOA, H ^{[1
]}

HEITZ, F ^{[1
]}

CALAS, B ^{[1
]}

GRIGORESCU, F ^{[1
]}

机构：

[1] CNRS,PHYSICOCHIM SYST POLYPHASES LAB,CNRS,URA 330,F-34033 MONTPELLIER,FRANCE

来源：

BIOORGANIC & MEDICINAL CHEMISTRY LETTERS | 1991年 / 1卷 / 06期

关键词：

D O I：

10.1016/S0960-894X(01)80812-X

中图分类号：

R914 [药物化学];

学科分类号：

100701 ;

摘要：

Using FmocTyrO(PO3Bzl2)OH, we synthesized and purified a triphosphosphorylated peptide corresponding to the major autophosphorylation site of the insulin receptor. By CD spectroscopy, conformational comparison of the phosphorylated peptide with its non-phosphorylated analogue showed a rigidification of the peptide backbone when the aromatic side chains bear negatively charged groups.

引用

页码：299 / 302

页数：4

共 10 条

[1]

BALEUX F, 1986, INT J PEPT PROT RES, V28, P22

[2] SOLID-PHASE SYNTHESIS USING A NEW POLYACRYLIC RESIN - SYNTHESIS OF THE FRAGMENT 14-21 OF THE AMINO-ACID SEQUENCE OF HISTONE H-4 [J].

CALAS, B ;

MERY, J ;

PARELLO, J ;

CAVE, A .

TETRAHEDRON, 1985, 41 (22) :5331-5339

[3]

CALAS B, 1985, INT BIOTECHNOLOGY LA, P18

[4] CONFORMATION OF POLY-L-TYROSINE IN TRIMETHYL PHOSPHATE SOLUTION [J].

DAMLE, VN .

BIOPOLYMERS, 1970, 9 (08) :937-&

[5]

KING DS, 1990, INT J PEPT PROT RES, V36, P255

[6]

MAGRE J, 1989, J CLIN ENDOCR METAB, V69, P1

[7] INTRINSIC KINASE-ACTIVITY OF THE INSULIN-RECEPTOR [J].

OHARE, T ;

PILCH, PF .

INTERNATIONAL JOURNAL OF BIOCHEMISTRY, 1990, 22 (04) :315-324

[8]

SATHYANARAYANA BK, 1986, INT J PEPT PROT RES, V27, P86

[9] SIGNAL TRANSDUCTION BY RECEPTORS WITH TYROSINE KINASE-ACTIVITY [J].

ULLRICH, A ;

SCHLESSINGER, J .

CELL, 1990, 61 (02) :203-212

[10]

WHITE MF, 1988, J BIOL CHEM, V263, P2969

← 1 →