POST-SYNTHETIC MODIFICATIONS OF NUCLEAR PROTEINS HIGH MOBILITY GROUP PROTEINS ARE METHYLATED

被引：54

作者：

BOFFA, LC

STERNER, R

VIDALI, G

ALLFREY, VG

机构：

[1] The Rockefeller University, New York

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1979年 / 89卷 / 04期

关键词：

D O I：

10.1016/0006-291X(79)92153-3

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Calf thymus high mobility group proteins 1 and 2 (HMG-1 and HMG-2) were purified to homogeneity from a 0.35 M NaCl extract of chromatin by selective precipitation with trichloroacetic acid followed by ion exchange chromatography on CM-Sephadex. Amino acid analysis of an acid hydrolyzate of HMG-1 and HMG-2 proteins revealed the presence of a ninhydrin-positive peak identified as NG,NG-dimethylarginine. Radioactive tracer experiments confirmed the presence of methylated arginine in HMG proteins. © 1979.

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页码：1322 / 1327

页数：6

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