3 GENES FOR PROTEIN PHOSPHATASE-1 MAP TO DIFFERENT HUMAN-CHROMOSOMES - SEQUENCE, EXPRESSION AND GENE LOCALIZATION OF PROTEIN SERINE/THREONINE PHOSPHATASE-1-BETA (PPP1CB)

被引：68

作者：

BARKER, HM

BREWIS, ND

STREET, AJ

SPURR, NK

COHEN, PTW

机构：

[1] UNIV DUNDEE, DEPT BIOCHEM, MRC, PROT PHOSPHORYLAT UNIT, DUNDEE DD1 4HN, SCOTLAND

[2] IMPERIAL CANC RES FUND, POTTERS BAR, HERTS, ENGLAND

来源：

BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH | 1994年 / 1220卷 / 02期

基金：

英国医学研究理事会;

关键词：

PROTEIN PHOSPHATASE; CDNA SEQUENCE; AMINO ACID SEQUENCE; MESSENGER-RNA ALTERNATIVE SPLICING; CHROMOSOMAL LOCALIZATION; (HUMAN);

D O I：

10.1016/0167-4889(94)90138-4

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Complementary DNA encoding a catalytic subunit of protein phosphatase 1, termed PP1 beta, was isolated from a human teratocarcinoma library. Hybridisation with different cDNA fragments showed that all human tissues examined contained 3.1 kb, 4.0 kb and 5.4 kb PP1 beta mRNAs arising from alternative splicing of the 3' noncoding region. The level of the 5.4 kb mRNA relative to the 3.1 kb mRNA was higher in skeletal muscle than in other tissues and the PP1 beta/PPl alpha mRNA ratio in rabbit tissues was highest in skeletal muscle. The 3' noncoding region of PP1 beta showed extreme conservation (greater than or equal to 90% identity) between man and rodents over 1.7 kb, suggesting that this region is of functional importance. The gene for human PP1 beta (PPP1CB) was localised to chromosome 2 by analysis of somatic cell hybrid DNA and mapped to band q23 by fluorescence in situ hybridisation. These data show that the genes for three protein phosphatase catalytic subunits PP1 alpha, PP1 beta, PP1 gamma are all located on different chromosomes.

引用

页码：212 / 218

页数：7

共 28 条

[1] INHIBITOR-2 FUNCTIONS LIKE A CHAPERONE TO FOLD 3 EXPRESSED ISOFORMS OF MAMMALIAN PROTEIN PHOSPHATASE-1 INTO A CONFORMATION WITH THE SPECIFICITY AND REGULATORY PROPERTIES OF THE NATIVE ENZYME [J].