TRANSLATION INITIATION FACTOR-DEPENDENT EXTRACTS FROM SACCHAROMYCES-CEREVISIAE

被引：36

作者：

ALTMANN, M

BLUM, S

PELLETIER, J

SONENBERG, N

WILSON, TMA

TRACHSEL, H

机构：

[1] UNIV BERN,INST BIOCHEM & MOLEK BIOL,BUHLSTR 28,CH-3012 BERN,SWITZERLAND

[2] MCGILL UNIV,DEPT MED,MONTREAL H3A 2T5,QUEBEC,CANADA

[3] MCGILL UNIV,MCGILL CANC CTR,MONTREAL H3A 2T5,QUEBEC,CANADA

[4] RUTGERS STATE UNIV,COOK COLL,CTR AGR MOLEC BIOL,NEW BRUNSWICK,NJ 08903

来源：

BIOCHIMICA ET BIOPHYSICA ACTA | 1990年 / 1050卷 / 1-3期

关键词：

Cap structure; Factor-dependent extract; Internal initiation; Translation initiation; Yeast;

D O I：

10.1016/0167-4781(90)90158-X

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Translation initiation factor 4A- and 4E-dependent extracts were developed from Saccharomyces cerevisiae and used to study factor requirements for translation of individual mRNAs in vitro. Whereas all mRNAs tested required eIF-4A, mRNAs devoid of secondary structure in their 5′ untranslated region did not require exogenous eIF-4E for translation. The latter included alfalfa mosaic virus RNA4, mRNA containing the untranslated region of tobacco mosaic virus RNA and mRNA containing part of the untranslated region of poliovirus RNA. Furthermore, initiation of translation on mRNAs containing part of the untranslated region of poliovirus RNA is most likely internal. © 1990.

引用

页码：155 / 159

页数：5

共 33 条

[1] PURIFICATION AND CHARACTERIZATION OF PROTEIN-SYNTHESIS INITIATION-FACTOR EIF-4E FROM THE YEAST SACCHAROMYCES-CEREVISIAE [J].