PROPROTEIN-PROCESSING ENDOPEPTIDASES OF THE INSULIN SECRETORY GRANULE

被引：30

作者：

BAILYES, EM

BENNETT, DL

HUTTON, JC

机构：

[1] Department of Clinical Biochemistry, University of Cambridge, Addenbrookes Hospital, Cambridge CB2 2QR, Hills Road

来源：

ENZYME | 1991年 / 45卷 / 5-6期

基金：

英国惠康基金;

关键词：

PROINSULIN; PROHORMONE CONVERTASE; ENDOPEPTIDASE; KEX-2; INSULIN GRANULE;

D O I：

10.1159/000468903

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Enzymological studies have implicated two Ca2+ dependent endopeptidases in the conversion of proinsulin to insulin: a type 1 activity and a type 2 activity which cleave on the C-terminal side of R31R32 and K64R65 in proinsulin, respectively. These activities were further characterized and their relationship to the mammalian family of subtilisin-like proteases was investigated. PC2 was expressed in neuroendocrine tissues and in insulinoma secretory granule fractions predominantly as a 65kDa protein. On anion-exchange chromatography of solubilized granules, PC1/3 immunoreactivity comigrated with a peak of type 1 activity whereas PC2 immunoreactivity coeluted with the peak of type 2 endopeptidase activity. PC2 antiserum gave a specific immunoprecipitation of type 2 activity from insulin granule extracts. It was concluded that the PC2 gene-product has type 2 endopeptidase activity.

引用

页码：301 / 313

页数：13

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