A NOVEL MEDIATOR BETWEEN ACTIVATOR PROTEINS AND THE RNA POLYMERASE-II TRANSCRIPTION APPARATUS

被引：365

作者：

KELLEHER, RJ

FLANAGAN, PM

KORNBERG, RD

机构：

[1] Department of Cell Biology Fairchild Center Stanford University School of Medicine Stanford

来源：

CELL | 1990年 / 61卷 / 07期

关键词：

D O I：

10.1016/0092-8674(90)90685-8

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

One gene activator protein may interfere with the effects of another in eukaryotic cells. We report here that a hybrid yeast-herpes gene activator protein inhibits transcriptional activation by a thymidine-rich DNA element in yeast. This example of activator interference can be faithfully reproduced in vitro. Interference is reversed by a partially purified yeast component, but not by RNA polymerase II or various polymerase II transcription factors. We conclude that the partially purified yeast component is a novel factor, and we suggest this factor mediates the transcriptional activation process. © 1990.

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页码：1209 / 1215

页数：7

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