SIMULTANEOUS MEASUREMENT OF CYTOCHROME P4501A CATALYTIC ACTIVITY AND TOTAL PROTEIN-CONCENTRATION WITH A FLUORESCENCE PLATE READER

被引：256

作者：

KENNEDY, SW

JONES, SP

机构：

[1] Environment Canada, Canadian Wildlife Service, National Wildlife Research Centre, Hull

来源：

ANALYTICAL BIOCHEMISTRY | 1994年 / 222卷 / 01期

关键词：

D O I：

10.1006/abio.1994.1476

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A method for simultaneous measurement of the cytochrome P4501A-associated enzyme, ethoxyresorufin-O-deethylase (EROD), and total protein concentration in liver microsomes is described. EROD assays were carried out in multiwell plates, and the fluorescent product (resorufin) and total proteins were quantified within the same wells with a fluorescence plate reader. Fluorescamine was used for protein assays. (C) 1994 Academic Press, Inc.

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页码：217 / 223

页数：7

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