MAPPING OF GENES-CONTROLLING LEISHMANIA-MAJOR INFECTION IN CXS RECOMBINANT INBRED MICE

Previous studies demonstrated that growth of the primary lesion following Leishmania major infection in inbred mice comes under the control of a single major gene designated Scl-1. Preliminary mapping studies had suggested a chromosome 8 location for the gene. In this paper a more detailed study of different disease phenotypes (lesion growth, splenomegaly, liver parasite load) in 14 CXS recombinant inbred (RI) mouse strains was undertaken in order to obtain a more definitive map location for the gene. Using the Kruskal-Wallis generalization of the Wilcoxon Rank-Sum Test to assign RI strains to parental phenotypes, high concordances with genes at the mid (Il-3) to distal end (Dlb-1, Hox-2, Sigje, Mtv-3 and Es-3) of chromosome 11 were demonstrated with two strains (LV39 and NIH173) of L. major given as promastigotes subcutaneously into the shaven rump. The results suggest that the most likely location for the previously described single major gene (Scl-1) regulating early lesion expansion is at the distal end of mouse chromosome 11, with the possibility that a gene located more proximally influences later phases of the infection.