SYNTHESIS OF PAPAIN IN ESCHERICHIA-COLI

被引：26

作者：

COHEN, LW ^{[1
]}

FLUHARTY, C ^{[1
]}

DIHEL, LC ^{[1
]}

机构：

[1] POMONA COLL,SEAVER LAB,CLAREMONT,CA 91711

来源：

GENE | 1990年 / 88卷 / 02期

关键词：

Carica papaya; protein processing; recombinant DNA; zymogen;

D O I：

10.1016/0378-1119(90)90041-O

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

We have transferred the cloned papain genetic information into an expression vector (pT7-7) regulated by the T7-promoter and have obtained in vitro expression as well as expression in Escherichia coli. In Western blots the proteins produced are immunologically recognizable as papain. Multiple forms of specific but differing sizes are detected, suggesting either that initiation can occur at more than one of the upstream methionines, or that the enzyme is processed after synthesis. © 1990.

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页码：263 / 267

页数：5

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