CLEAVAGE OF SINGLE-STRANDED-DNA BY THE A AND A-STAR PROTEINS OF BACTERIOPHAGE-PI-X174

被引：42

作者：

LANGEVELD, SA ^{[1
]}

VANMANSFELD, ADM ^{[1
]}

WINTER, JMD ^{[1
]}

WEISBEEK, PJ ^{[1
]}

机构：

[1] STATE UNIV UTRECHT,PHYSIOL CHEM LAB,UTRECHT,NETHERLANDS

来源：

NUCLEIC ACIDS RESEARCH | 1979年 / 7卷 / 08期

关键词：

D O I：

10.1093/nar/7.8.2177

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The purified A protein and A* protein of bacteriophage φX174 have been tested for endonucleaae activity on single stranded viral φX174 DNA. The A protein (55.000 daltons) nicks single-stranded DNA in the same way and at the same place as it does superhelical RFI DNA, at the origin of DNA replication. The A* protein (37.000 daltons) can cleave the single-stranded viral DNA at many different sites. It has however a strong preference for the origin of replication. Both proteins generate 3′OH ends and blocked 5′ termini at the nick site. © 1979 Information Retrieval Limited.

引用

页码：2177 / 2188

页数：12

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