CHARACTERIZATION OF THE GENE FOR CHROMOSOMAL TRIMETHOPRIM-SENSITIVE DIHYDROFOLATE-REDUCTASE OF STAPHYLOCOCCUS-AUREUS ATCC 25923

被引：25

作者：

DALE, GE

THEN, RL

STUBER, D

机构：

[1] F HOFFMANN LA ROCHE & CO LTD,DEPT NEW TECHNOL & INFECT DIS,CH-4002 BASEL,SWITZERLAND

[2] F HOFFMANN LA ROCHE & CO LTD,DEPT PHARMACEUT RES,CH-4002 BASEL,SWITZERLAND

来源：

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY | 1993年 / 37卷 / 07期

关键词：

D O I：

10.1128/AAC.37.7.1400

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The gene for the trimethoprim-sensitive (Tmp(s)) chromosomal dihydrofolate reductase (DHFR) of Staphylococcus aureus ATCC 25923 was cloned and characterized. The structural gene encodes a polypeptide of 159 amino acid residues and has a calculated molecular weight of 18,251. The amino acid sequences of this Tmp(s) DHFR and those of the trimethoprim-resistant type S1 DHFR encoded by transposon Tn4003 are 80% identical. In contrast to the trimethoprim-resistant enzyme, the Tmp(s) DHFR can be highly overexpressed in Escherichia coli, with most of the recombinant protein occurring in a soluble and an active form.

引用

页码：1400 / 1405

页数：6

共 25 条

[1] DIHYDROFOLATE-REDUCTASE HYSTERESIS AND ITS EFFECT ON INHIBITOR BINDING ANALYSES [J].