CONVERSION OF PORCINE BIG ENDOTHELIN TO ENDOTHELIN BY AN EXTRACT FROM THE PORCINE AORTIC ENDOTHELIAL-CELLS

被引:121
作者
MATSUMURA, Y
IKEGAWA, R
TAKAOKA, M
MORIMOTO, S
机构
[1] Department of Pharmacology, Osaka University of Pharmaceutical Sciences, Matsubara, Osaka, 580
关键词
D O I
10.1016/0006-291X(90)91751-D
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Conversion of porcine big endothelin (big ET) to endothelin (ET) by an extract from cultured porcine aortic endothelial cells was investigated using a radioimmunoassay (RIA) specific for ET and reverse-phase high performance liquid chromatography (RP-HPLC). When big ET was incubated with the extract at an acid pH in the presence of E-64, a cysteine protease inhibitor, the amount of immunoreactive-ET (IR-ET) in the incubation mixture was greatly increased and the optimum pH for this increased reaction was 4.0. The extract-induced increase in IR-ET was completely inhibited by pepstatin-A. These immunoreactive alterations correlated with those in the vasoconstrictor activity. When the incubation mixture of big ET with the cell extract was applied to the RP-HPLC, the IR-ET eluted at the same retention time as seen with synthetic porcine ET. We suggest that a pepstatin-sensitive aspartic protease is involved in the conversion of big ET to ET in vascular endothelial cells. © 1990.
引用
收藏
页码:203 / 210
页数:8
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