SPECIFIC BINDING OF ESTROGEN TO OSTEOCLAST SURFACES

被引：45

作者：

BRUBAKER, KD ^{[1
]}

GAY, CV ^{[1
]}

机构：

[1] PENN STATE UNIV,DEPT POULTRY SCI,UNIV PK,PA 16802

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1994年 / 200卷 / 02期

关键词：

D O I：

10.1006/bbrc.1994.1535

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Specific plasma membrane binding sites for 17 beta-estradiol were shown to exist on avian osteoclasts by the use of a fluorescent conjugated estrogen, 17 beta-estradiol -6- carboxymethyloxime- bovine serum albumin fluorescein isothiocyanate. Binding was dose and time dependent, as well as specific, since 17 beta-estradiol blocked the binding of the complex. Tamoxifen was also an effective blocking agent. Rapid responses to 17 beta-estradiol included decreased acid production (41.5%) and distinct cell shape changes. (C) 1994 Academic Press, Inc.

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页码：899 / 907

页数：9

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