A NEW CONUS PEPTIDE LIGAND FOR MAMMALIAN PRESYNAPTIC CA2+ CHANNELS

被引：463

作者：

HILLYARD, DR

MONJE, VD

MINTZ, IM

BEAN, BP

NADASDI, L

RAMACHANDRAN, J

MILJANICH, G

AZIMIZOONOOZ, A

MCINTOSH, JM

CRUZ, LJ

IMPERIAL, JS

OLIVERA, BM

机构：

[1] UNIV UTAH,DEPT PATHOL,SALT LAKE CITY,UT 84132

[2] UNIV UTAH,DEPT PSYCHIAT,SALT LAKE CITY,UT 84132

[3] HARVARD UNIV,SCH MED,DEPT NEUROBIOL,BOSTON,MA 02115

[4] UNIV PHILIPPINES,INST CHEM,QUEZON CITY 1107,PHILIPPINES

[5] UNIV PHILIPPINES,INST MARINE SCI,QUEZON CITY 1107,PHILIPPINES

[6] NEUREX CORP,MENLO PK,CA 94025

来源：

NEURON | 1992年 / 9卷 / 01期

关键词：

D O I：

10.1016/0896-6273(92)90221-X

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

Voltage-sensitive Ca2+ channels that control neurotransmitter release are blocked by omega-conotoxin (omega-CgTx) GVIA from the marine snail Conus geographus, the most widely used inhibitor of neurotransmitter release. However, many mammalian synapses are omega-CgTx-GVIA insensitive. We describe a new Conus peptide, omega-CgTx-MVIIC, that is an effective inhibitor of omega-CgTx-GVIA-resistant synaptic transmission. Ca2+ channel targets that are inhibited by omega-CgTx-MVIIC but not by omega-CgTx-GVIA include those mediating depolarization-induced Ca-45(2+) uptake in rat synaptosome preparations, "P" currents in cerebellar Purkinje cells, and a subset of omega-CgTx-GVIA-resistant currents in CA1 hippocampal pyramidal cells. The characterization of omega-CgTx-MVIIC by a combination of molecular genetics and chemical synthesis defines a general approach for obtaining ligands with novel receptor subtype specificity from Conus.

引用

页码：69 / 77

页数：9

共 24 条

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