ANALYSIS OF PHENYLTHIOHYDANTOIN AMINO-ACIDS BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY ON DUPONT ZORBAX CYANOPROPYLSILANE COLUMNS

被引：113

作者：

JOHNSON, ND

HUNKAPILLER, MW

HOOD, LE

机构：

[1] Division of Biology, California Institute of Technology, Pasadena

来源：

ANALYTICAL BIOCHEMISTRY | 1979年 / 100卷 / 02期

基金：

美国国家卫生研究院;

关键词：

D O I：

10.1016/0003-2697(79)90237-9

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The phenylthiohydantoins (Pth) of the common amino acids can be resolved in a single analysis using a 25 × 0.46-cm DuPont Zorbax cyanopropylsilane (CN) column developed with a gradient of methanol/acetonitrile (17:3) in sodium acetate buffer, pH 5.4. The Zorbax CN columns exhibit greater durability, reproducibility, and sensitivity than do columns with an octadecylsilane (C18) support when used for Pth amino acid analysis in automated polypeptide sequencing. © 1979.

引用

页码：335 / 338

页数：4

共 8 条

[1]

BROWN AS, 1978, J CHROMATOGR, V148, P532

[2] A PROTEIN SEQUENATOR [J].