AN EFFECTIVE TRANSFORMATION METHOD FOR HANSENULA-POLYMORPHA

被引：37

作者：

BERARDI, E ^{[1
]}

THOMAS, DY ^{[1
]}

机构：

[1] BIOTECHNOL RES INST,GENET ENGN SECT,MONTREAL H4P 2R2,QUEBEC,CANADA

来源：

CURRENT GENETICS | 1990年 / 18卷 / 02期

关键词：

Hansenula polymorpha; LEU2; Methylotrophic yeasts; Thermotolerant yeasts; Transformation;

D O I：

10.1007/BF00312606

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

We have developed a rapid, simple transformation procedure for intact cells of Hansenula polymorpha. It is a modification of the LiAc method and can yield 104-105 transformants/μg DNA. The use of stationaryphase cells, a high cell density per plate and a hear pulse at 50°C for 10 min are among major modifications of the original method. We have also found that, within the XhoI-SalI chromosomal fragment of Saccharomyces cerevisae containing the LEU2 gene, a sequence exists which supports autonomous replication of plasmid moleculles in H. polymorpha. © 1990 Springer-Verlag.

引用

页码：169 / 170

页数：2

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