HUMAN-LYMPHOCYTE ANTIGENS - PRODUCTION OF A MONOCLONAL ANTIBODY THAT DEFINES FUNCTIONAL THYMUS-DERIVED LYMPHOCYTE SUBSETS

被引：273

作者：

HAYNES, BF ^{[1
]}

EISENBARTH, GS ^{[1
]}

FAUCI, AS ^{[1
]}

机构：

[1] NHLBI,BIOCHEM GENET LAB,BETHESDA,MD 20205

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 1979年 / 76卷 / 11期

关键词：

D O I：

10.1073/pnas.76.11.5829

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

A monoclonal mouse antibody (3A1) that specifically bound to 65% of human peripheral blood (PB) thymus-derived (T) cells but did not bind to complement receptor-positive PB bone marrow-derived (B) cells, polymorophonuclear leukocytes, or human erythrocytes has been produced. The 3A1 antibody was synthesized by a stable cloned lymphocyte hybrid cell line. This lymphocyte hybrid line (3A1) was derived from fusion of P3x63/Ag8 myeloma cells and spleen cells from BALB/c mice immunized with HSB-2 cells, a human T cell line. The 3A1 lymphocyte hybrid line produced mouse ascites fluid containing 3A1 antibody in saturating titers of up to 1:25,600. Purified PB T cells that carried the 3A1 antigen incorporated tritiated thymidine maximally in response to phytohemagglutinin and concanavalin A stimulation, whereas purified PB T cells that lacked the 3A1 antigen responded suboptimally to phytohemagglutinin and minimally to concanavalin A. Thus, the 3A1 antibody can be easily used to study the role of 3A1-positive and negative T cell subsets in the regulation of normal and abnormal human immune responses.

引用

页码：5829 / 5833

页数：5

共 32 条

[1]

ADAMS RA, 1968, CANCER RES, V28, P1121

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