HISTAMINE-H-3 RELEASE FROM HUMAN-LEUKOCYTES - NEW METHOD FOR DETECTING TYPE-I ALLERGY COMPARED WITH BASOPHIL HISTAMINE-RELEASE TECHNIQUE

被引：13

作者：

SKOV, PS ^{[1
]}

NORN, S ^{[1
]}

WEEKE, B ^{[1
]}

机构：

[1] RIGSHOSP, DEPT MED TA, DIV CLIN IMMUNOL, CLIN ALLERGOL LAB, DK-2100 COPENHAGEN, DENMARK

来源：

ALLERGY | 1979年 / 34卷 / 04期

关键词：

allergen; histamine release; in vitro; leukocytes; simple method;

D O I：

10.1111/j.1398-9995.1979.tb01707.x

中图分类号：

R392 [医学免疫学];

学科分类号：

100102 ;

摘要：

A rapid, simple, and inexpensive method for large scale screening of patients suspected of type I allergy has been developed. The method is based on in vitro incorporation of 3H‐histamine in the leukocytes of the patient, whereafter release of labelled histamine is measured after provocation of the cells with the suspected allergen. The new method was compared with the conventional basophil histamine release technique by in vitro provocation of six asthmatic patients under suspicion of type 1 allergy against animal dander, house dust, and mite, and an almost identical it‐lease of histamine was observed in both assays. Copyright © 1979, Wiley Blackwell. All rights reserved

引用

页码：261 / 263

页数：3

共 5 条

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[2] SPECIFIC DIAGNOSIS OF EXOGENOUS BRONCHIAL-ASTHMA IN ADULTS - COMPARISON BETWEEN CASE HISTORY, INTRACUTANEOUS TEST, RAST, HISTAMINE-RELEASE FROM BASOPHIL LEUKOCYTES, AND BRONCHIAL PROVOCATION [J].

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MALLING, HJ ;

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ALLERGY, 1979, 34 (03) :175-185

[3]

SHORE PA, 1959, J PHARMACOL EXP THER, V127, P182

[4] SIMPLIFIED METHOD FOR MEASURING BASOPHIL HISTAMINE-RELEASE AND BLOCKING ANTIBODIES IN HAY-FEVER PATIENTS - BASOPHIL HISTAMINE CONTENT AND CELL PRESERVATION [J].

SKOV, PS ;

NORN, S .

ACTA ALLERGOLOGICA, 1977, 32 (03) :170-182

[5]

STAHLSKOV P, 1977, CLIN EXP IMMUNOL, V27, P432

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